Protein_Domain

Part:BBa_K588101:Design

Designed by: Kevin Lu   Group: iGEM11_Rutgers   (2011-09-27)

C-terminal leucine zipper domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cloned from TU#711.

Original zipper had 1 PstI site. It was removed (via silent mutation) by mismatches in PCR primers

Original Sequence 5'- CAGCTGGAGAAGAAACTGCAGGCTCTGGAGAAGAAACTGGCACAGCTGGAGTGGAAAAACCAGGCACTGGAGAAGAAACTGGCAC -3'
Mutated Sequence 5'- CAGCTGGAGAAGAAACTTCAGGCTCTGGAGAAGAAACTGGCACAGCTGGAGTGGAAAAACCAGGCACTGGAGAAGAAACTGGCAC -3'

Added RFC25 prefix and suffix

Forward Primer 5'-cgactgGAATTCCGCGGCCGCTTCTAGAGATGGCCGGC CAGCTGGAGAAGAAACTTCAGGC-3' Reverse Primer 5'- cgactgCTGCAGCGGCCGCTCTACTAGTATTAACCGGT GTGCCAGTTTCTTCTCCAG-3'

Source

TU#711 provided by Monica Driscoll and described in Zhang et al.

References

Zhang, Shifang, Charles Ma, and Martin Chalfie. Combinatorial Marking of Cells and Organelles with Reconstituted Fluorescent Proteins. Cell 119. (2004) : 137-144